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Co-Authors
- Rahul C. Salunkhe
- Dhiraj P. Dhotre
- Bipinchandra K. Salunke
- Vikas S. Patil
- Vaibhav Mahale
- Raymond J. Andrew
- Milind S. Patole
- Ketan P. Narkhede
- Rohit Sharma
- Ashish V. Polkade
- Swapnil Sopan Gaikwad
- Hitendra Munot
- Md. Imran
- Bhukya Saida
- Sanjeev C. Ghadi
- Pankaj Verma
- Vikas C. Ghattargi
- Mangesh V. Suryavanshi
- Swapnil Kalam
- Bharati S. Meti
- Mani Garg
- Sudeeksha Raina
- Jovita D. Silva
- Shrikant P. Pawar
- Praveen Rahi
- Om Prakash
- Avinash Sharma
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Shouche, Yogesh S.
- Distribution and Molecular Characterization of Wolbachia Endosymbionts in Odonata (Insecta) from Central India by Multigene Approach
Abstract Views :287 |
PDF Views:112
Authors
Rahul C. Salunkhe
1,
Dhiraj P. Dhotre
2,
Bipinchandra K. Salunke
1,
Vikas S. Patil
2,
Vaibhav Mahale
1,
Raymond J. Andrew
3,
Milind S. Patole
1,
Ketan P. Narkhede
4,
Yogesh S. Shouche
2
Affiliations
1 National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, IN
2 Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, IN
3 Department of Zoology, Hislop College, Nagpur 440 001, IN
4 Department of Microbiology, Moolji Jaitha College, Jalgaon 425 002, IN
1 National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, IN
2 Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, IN
3 Department of Zoology, Hislop College, Nagpur 440 001, IN
4 Department of Microbiology, Moolji Jaitha College, Jalgaon 425 002, IN
Source
Current Science, Vol 108, No 5 (2015), Pagination: 971-978Abstract
Wolbachia are maternally inherited bacterial endo-symbionts of arthropods distributed among a wide range of hosts. It is now well known that they induce reproductive manipulations in their arthropod hosts by various phenotypic effects. The objective of the present study was to investigate Wolbachia infection among the insect order Odonata comprising 16 species from 5 families. Fifteen odonate species representing five families were found to harbour Wolbachia with the overall infection rate of 70%, out of which four-teen species are reported for the first time. According to multilocus sequence typing (MLST) data and phylo-genetic analysis, all odonate Wolbachia species belong to supergroup F, except Trithemis pallindinervis, which belongs to supergroup B. MLST data reveal 20 new, highly similar STs (99.32 0.34). We found a high rate of Wolbachia infection in Odonata of India, which indicates importance of this association. The charac-terization of these Wolbachia strains promises to lead to a deeper insight into this interaction, which is essential for further studies based on their phenotypic effects. The study suggests that all the characterized Wolbachia STs are totally new and arise as a result of point mutation.Keywords
Multilocus Sequence Typing, Phenotypic Effects, Point Mutations.- 'Species Concept' in Microbial Taxonomy and Systematics
Abstract Views :814 |
PDF Views:158
Authors
Affiliations
1 Microbial Culture Collection, National Centre for Cell Science, Ganeshkhind, Pune 411 007, IN
1 Microbial Culture Collection, National Centre for Cell Science, Ganeshkhind, Pune 411 007, IN
Source
Current Science, Vol 108, No 10 (2015), Pagination: 1804-1814Abstract
Species is the basic unit of identification of a large diversity of organisms. The concept of species is old and has undergone several changes depending on the kind of organism(s). Earlier concepts were formulated considering mostly macroorganisms (plants or animals). In 21st century the microbial species concept took shape which changed from morphological, ecological, biological and phylogenetic to more agreeable polyphasic species concept. Polyphasic species concept involves a combination of characters such as morphology, physiology, biochemical markers and sequencing of various regions of the DNA known as molecular markers. There are also specific criteria such as DNA-DNA hybridization for complex genera of Bacteria and Archaea, extrolite profiling for complex genera of fungi, etc. Although species concept and taxonomy of Bacteria are more systematic than fungi, taxonomy in two domains (bacteria, archaea) and fungi of eukarya domain is undergoing changes. The time is not far when genome sequencing will be considered as another criterion for delineating species. This article also discusses the role of various techniques (MALDI, FAME, etc.) in species identification. The concept of Candidatus species and species annotation for metagenomic studies is also discussed. We briefly describe the criteria used to delineate species in various microbes and the need for different species concepts based on the kind of organism.Keywords
Archaea, Bacteria, Fungi, Species Concept, Taxonomy and Systematics.- Utility of DNA Barcoding for Identification of Bird-Strike Samples from India
Abstract Views :447 |
PDF Views:121
Authors
Affiliations
1 Microbial Culture Collection, First Floor, Central Tower, Sai Trinity Building, Garware Circle, Sutarwadi, Pashan, Pune 411 021, IN
1 Microbial Culture Collection, First Floor, Central Tower, Sai Trinity Building, Garware Circle, Sutarwadi, Pashan, Pune 411 021, IN
Source
Current Science, Vol 110, No 1 (2016), Pagination: 25-28Abstract
No Abstract.- The Gut-Associated Klebsiella Sp. of the Apple Snail Produces Multiple Polysaccharide Degrading Enzymes
Abstract Views :311 |
PDF Views:122
Authors
Affiliations
1 Department of Biotechnology, Goa University, Taleigao Plateau, Goa 403 206, IN
2 National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, IN
3 Department of Biotechnology, Goa University, Taleigao Plateau, Goa 403 206,, IN
4 National Centre for Cell Sciences, Ganeshkhind, Pune 411 007, IN
1 Department of Biotechnology, Goa University, Taleigao Plateau, Goa 403 206, IN
2 National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, IN
3 Department of Biotechnology, Goa University, Taleigao Plateau, Goa 403 206,, IN
4 National Centre for Cell Sciences, Ganeshkhind, Pune 411 007, IN
Source
Current Science, Vol 110, No 11 (2016), Pagination: 2170-2172Abstract
Pila globosa, an edible variety of apple snail is a common inhabitant of lentic ecosystem and feeds on plant detritus. The tissue extract of gastrointestinal tract from Pila globosa demonstrated the presence of carboxymethyl cellulase, xylanase, alginate lyase and pectinase activity. Culture-dependent method was used to isolate carboxymethyl cellulose (CMC) degrading bacteria from the gastrointestinal tract of apple snail. Morphologically identical colonies were obtained on M9 gelrite plates containing CMC as carbon source. One such bacterial isolate was purified by streaking and designated as strain PG-1. Bacterial strain PG-1 degraded CMC, pectin, starch and alginate. The 16S rDNA sequence of strain PG1 was 99% identical to Klebsiella oxytoca. Phylogenetic analysis using maximum likelihood revealed the clustering of strain PG1 with the clade belonging to Klebsiella oxytoca type strain.Keywords
Apple Snail, Klebsiella, Pila globosa, Polysaccharide Degrading Bacteria.- Need to Explore Camel Milk as Probiotics
Abstract Views :725 |
PDF Views:143
Authors
Vikas C. Ghattargi
1,
Mangesh V. Suryavanshi
1,
Swapnil Kalam
1,
Yogesh S. Shouche
1,
Bharati S. Meti
2
Affiliations
1 National Centre for Microbial Resource, National Centre for Cell Science, Central Tower, Pashan, Pune 411 021, IN
2 Department of Bio-Technology, Basaveshwar Engineering College, Bagalkot 587 102, IN
1 National Centre for Microbial Resource, National Centre for Cell Science, Central Tower, Pashan, Pune 411 021, IN
2 Department of Bio-Technology, Basaveshwar Engineering College, Bagalkot 587 102, IN
Source
Current Science, Vol 114, No 03 (2018), Pagination: 425-426Abstract
Consumption of probiotics is evidently on the rise globally because of their various health benefits on human beings. The search for novel probiotics with diverse properties is the necessity of time. Properties of camel milk were first mentioned in the ‘Words of The Prophet Mohammed’ in the Surah, a section of the Koran (volume 7, book 71, number 5909).References
- Yadav, A. K., Kumar, R., Priyadarshini, L. and Singh, J., Asian J. Dairy Food Res., 2015, 34(2), 83.
- Khedid, K., Faid, M., Mokhtari, A., Soulaymani, A. and Zinedine, A., Microbiol. Res., 2009, 164(1), 81–91.
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- Assessment of Airborne Microbial Community in Indian Cities during the Middle East Dust Storm
Abstract Views :311 |
PDF Views:102
Authors
Vikas C. Ghattargi
1,
Mani Garg
1,
Sudeeksha Raina
1,
Jovita D. Silva
1,
Shrikant P. Pawar
1,
Praveen Rahi
1,
Yogesh S. Shouche
1
Affiliations
1 National Centre for Microbial Resource, National Centre for Cell Science, Pune 411 007, IN
1 National Centre for Microbial Resource, National Centre for Cell Science, Pune 411 007, IN
Source
Current Science, Vol 117, No 10 (2019), Pagination: 1693-1700Abstract
Globally dust storms have greatly affected air quality, and nearly 5.0 billion tonnes of dust undergoes migra-tion every year. A plethora of microorganisms spread far and wide along with the dust particles during dust storms. In April 2015, a dust storm originated from the Middle East and travelled to many Indian cities. We analysed the impact of the dust storm on the diversity and composition of aerial microorganisms using cultivation-based methods. Sampling was done in three cities (Mumbai, Lonavala and Pune) during a dust storm day (DSD) and a post-dust storm day (PSD). A total of 580 morphologically different bacte-ria and then ten mycelial fungi were isolated during the study. Identification based on MALDI-TOF MS biotyping, sequencing of 16S rRNA gene and ITS region revealed that the bacterial isolates belonged to 32 different genera and fungi to four different genera. Principal coordinate analysis exhibited separate grouping of DSD and PSD samples, indicating the shift in microbial communities. Osmotolerant and halotolerant bacterial genera, viz. Psychrobacter and Exiguobacterium were recorded specifically during DSD. The proportion of opportunistic pathogens, including Staphylococcus and Enterobacter was high during DSD in comparison to PSD. Overall, the study reveals the influence of dust storms on the aerial microbial composition and indicates the possible spread of specific microbial species during a dust storm event.Keywords
Airborne, Dust Storm, High-Throughput Cultivation, Microorganisms, Mass Spectroscopy.References
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- Importance of Vision, Visibility and Viability of Microbial Resource Centres
Abstract Views :337 |
PDF Views:113
Authors
Affiliations
1 National Centre for Microbial Resource, National Centre for Cell Science, Pune 411 007, IN
1 National Centre for Microbial Resource, National Centre for Cell Science, Pune 411 007, IN